The effects of cyclical simulation on the axon hillock diameter of murine intracortical neurons
نویسندگان
چکیده
Changes to the axon hillock in frequently firing neurons are known to be important predictors of early disease states. Studying this phenomenon is critical to understanding the first insult implicated in multiple neuro-degenerative disorders. To study these changes we used cyclical stimulations using micro-electrodes to the axon hillock of mouse intracortical neurons. Numerical simulation results indicate that axon hillock water potential fluctuated sinusoidally on high voltage only. Fluctuations in the amplitude and trend were caused by calcium flow and storage resistance, respectively. The change in axon hillock-stored water was proportional to the change rate in water potential. Axon hillock diameter increased with fluctuations in calcium free media; moreover, it varied slightly under low voltage conditions. Changes in axon hillock diameter were caused by changes in water potential, which was determined by subcellular gated channels, media calcium potential, and other baseline characteristics of neurons.
منابع مشابه
The Effect of Noscapine on Oxygen-Glucose Deprivation on Primary Murine Cortical Neurons in High Glucose Condition
AbstractIn the present work we set out to investigate the neuroprotective effects of noscapine (0.5-2 µM) in presence of D-glucose on primary murine foetal cortical neurons after oxygen–glucose deprivation/24 hrs recovery. Cell viability, nitric oxide production and intracellular calcium ([ca2+]i) levels were evaluated by MTT assay, the modified Griess method and Fura-2 respectively. 25 and 100...
متن کاملThe Effect of Noscapine on Oxygen-Glucose Deprivation on Primary Murine Cortical Neurons in High Glucose Condition
AbstractIn the present work we set out to investigate the neuroprotective effects of noscapine (0.5-2 µM) in presence of D-glucose on primary murine foetal cortical neurons after oxygen–glucose deprivation/24 hrs recovery. Cell viability, nitric oxide production and intracellular calcium ([ca2+]i) levels were evaluated by MTT assay, the modified Griess method and Fura-2 respectively. 25 and 100...
متن کاملSpike Response Fitting and Prediction of a Single Neuron Model
Conductance based detail biological models of neurons are able to predict various forms of spiking patterns with great accuracy, but the computational resources required to simulate large network of such neurons are still incomprehensible. As an alternative various simplified spiking models of neurons have been proposed. These models achieve computational efficiency through dynamical system tec...
متن کاملHeterogeneity of the Axon Initial Segment in Interneurons and Pyramidal Cells of Rodent Visual Cortex
The microdomain that orchestrates action potential initiation in neurons is the axon initial segment (AIS). It has long been considered to be a rather homogeneous domain at the very proximal axon hillock with relatively stable length, particularly in cortical pyramidal cells. However, studies in other brain regions paint a different picture. In hippocampal CA1, up to 50% of axons emerge from ba...
متن کاملTwo mRNA-binding proteins regulate the distribution of syntaxin mRNA in Aplysia sensory neurons.
Targeting mRNAs to different functional domains within neurons is crucial to memory storage. In Aplysia sensory neurons, syntaxin mRNA accumulates at the axon hillock during long-term facilitation of sensory-motor neuron synapses produced by serotonin (5-HT). We find that the 3' untranslated region of Aplysia syntaxin mRNA has two targeting elements, the cytosolic polyadenylation element (CPE) ...
متن کامل